Composite

Part:BBa_K091126:Design

Designed by: Pallavi Penumetcha   Group: iGEM08_Davidson-Missouri_Western   (2008-07-10)


pTet regulated production of LacI_X86


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In designing the mutation, we chose the amino acid that would represent leucine by deducing which leucine amino acid was most common in the rest of the LacI gene sequence.


Source

The basic sequence for the LacI gene sequence was obtained from Ecogene.com.

References